Incubator Accessories
Diagnostic Biosystems BCL-10 Bcl-10
BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFĪŗB activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.
Diagnostic Biosystems LYSOZYME Lysozyme
This antibody reacts with lysozyme. It stains granulocytes, monocytes and macrophages in human tonsil, skin and colon. This antibody does not crossreact with any other cell types.
Diagnostic Biosystems CD44 H CAM CD44 /HCAM
This antibody is specific to a cell surface glycoprotein of 80‐95 kDa, known as CD44. CD44 is expressed on the surface of lymphocytes, monocytes and granulocytes. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Diagnostic Biosystems IGG-FITC IgG-FITC
This antibody reacts with the gamma chain of human IgG. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.
Diagnostic Biosystems PD-1 PD-1
The programmed death receptor 1 (PD-1) protein is a cell-surface receptor on certain lymphocytes that, with its ligand programmed death ligand 1 (PD-L1), helps to down-regulate immune responses. Many cancer types express PD-L1 and evade immune recognition via the PD-1/PD-L1 interaction. Precision therapies targeting the PD-1/PD-L1 pathway have the potential to improve response and thereby offer a novel treatment avenue to some patients with cancer.
Diagnostic Biosystems AFB AFB
This kit stains acid-fast bacteria and tubercle bacilli of Mycobacterium genus in paraffin tissue sections. Tubercle bacilli and other acid-fast bacteria stain bright red and the background stains light green.
Diagnostic Biosystems IRON STAIN Iron Stain
The Iron Stain Kit is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis. This product is based on the Prussian Blue reaction in which ionic iron reacts with acid ferrocyanide producing a blue color.
Diagnostic Biosystems ELASTIC Elastic
The Elastic Stain Kit (Modified Verhoff’s) is intended for use in histological demonstration of elastin in tissue sections. Demonstration of elastic tissue is useful in cases of emphysema (atrophy of elastic tissue), arteriosclerosis (thinning and loss of elastic fibers) and various other vascular diseases.
Diagnostic Biosystems HER2/NEU C-ERBB-2 c-erbB-2 Oncoprotein
This antibody reacts with the c-erbB-2 oncoprotein, a 190 kDa protein. The proto-oncogene for c-erbB-2 islocated on the human chromosome 17, band 21. It has structural similarities to EGFR. Several studies have indicated that c-erbB-2 may be a good indicator of the prognosis of human carcinomas in the breast, ovary, uterus and gastrointestinal tract.
Diagnostic Biosystems PERMARED/AP-AUTO PermaRed/AP
PermaRed/AP is a substrate-chromogen system designed to be used for either IHC or ISH when using alkaline phosphatase detection. PermaRed/AP produces a brilliant dark red color. PermaRed/AP is insoluble in organic solvents; therefore, sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use.
Diagnostic Biosystems MOC-31 MOC-31
MOC-31 antibody recognizes an epithelial-associated, glycoprotein located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells. It is not present in most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. MOC31 may be used in a panel of antibodies as a negative marker for mesothelioma, or lung adenocarcinoma. Studies have shown that MOC-31 is useful in differentiating tumors of unknown origin in liver cancers and distinguishing cholangiocarcinoma from hepatocellular carcinomas
Diagnostic Biosystems SMOOTH MUSCLE MYOSIN Smooth Muscle Myosin (SMMS-1)
Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2, they are differentially expressed depending on the functional activity of the muscle.\nThe antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors
Diagnostic Biosystems GMS GMS
The Modified Gomori Methenamine-Silver Nitrate Stain (GMS Stain Kit) is intended for use in the histologic visualization of fungi, basement membrane and some opportunistic organisms such as Pneumocystis carinii. Pneumocystis carinii is an opportunistic pathogen that causes severe pulmonary disease in humans, dogs, rats, mice and other vertebrate species with acquired, induced, or inherited immune deficiency syndromes. In addition, this procedure will demonstrate Actinomyces and related species, Nocardia asteroids, and certain encapsulated bacteria.
Diagnostic Biosystems Solution Pepsin
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens.\n\nFixation is one of the most critical aspects of immunostaining. If the antigen is not properly fixed it will be washed out of the specimen. On the other hand, overfixation can cause severe problems such as masking or denaturation of the antigen. Formaldehyde is probably the best all-around fixative, due to its cross-linking characteristics. Length of fixation is very critical to prevent antigen masking.